Screening novel genes by a comprehensive strategy to construct multiple stress-tolerant industrial Saccharomyces cerevisiae with prominent bioethanol production

Table 2 Comparisons of fermentation performance of engineered strains using resistant strain E-158 as the host

Indexes	Strains	8.0% (v/v) Initial ethanol (YPD150)	44 °C (YPD100)	43 °C + 2.6% (v/v) Initial ethanol (YPD100)	28% Glucose (YPD280)	1.5 M NaCl (YPD150)
Final generated ethanol concentration (g/L)	E-158	28.64 ± 1.66bc	38.12 ± 1.90b	18.66 ± 1.62b	128.40 ± 1.17b	28.13 ± 1.58c
	E-158-CRZ1	25.24 ± 1.99c	42.64 ± 1.71a	24.34 ± 1.76a	131.77 ± 0.79a	46.90 ± 2.10b
	E-158-ENA5	32.39 ± 1.02a	43.52 ± 1.17a	23.43 ± 0.95a	132.90 ± 1.80a	56.73 ± 1.05a
	E-158	78.00 ± 3.40a	17.91 ± 1.72a	56.25 ± 3.00a	25.75 ± 2.22a	72.47 ± 4.53a
Residual glucose (g/L)	E-158-CRZ1	82.34 ± 2.35a	9.70 ± 2.66b	43.99 ± 3.30b	19.21 ± 1.95b	34.24 ± 4.36b
Residual glucose (g/L)	E-158-ENA5	70.44 ± 1.74b	7.21 ± 2.03b	44.13 ± 1.40b	17.79 ± 2.79b	8.75 ± 2.54c
Ethanol yield (g ethanol/g consumed glucose)	E-158	0.45 ± 0.01ab	0.46 ± 0.00a	0.44 ± 0.00a	0.50 ± 0.00a	0.40 ± 0.01b
	E-158-CRZ1	0.43 ± 0.01b	0.47 ± 0.01a	0.44 ± 0.01a	0.50 ± 0.01a	0.43 ± 0.00a
	E-158-ENA5	0.46 ± 0.01a	0.47 ± 0.01a	0.43 ± 0.01a	0.50 ± 0.01a	0.42 ± 0.01a

The data in the table are those at the end of fermentation. Values indicate mean ± standard deviation of three biological replications. Values followed by different lowercase letters in the same column indicate significant differences at the level of P < 0.05 (Tukey-test) among strains. Same lowercase letters, no difference. E-158-CRZ1: Overexpression of TF Crz1p in E-158; E-158-ENA5: Overexpression of ENA5 in E-158

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