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Table 2 Comparisons of fermentation performance of engineered strains using resistant strain E-158 as the host

From: Screening novel genes by a comprehensive strategy to construct multiple stress-tolerant industrial Saccharomyces cerevisiae with prominent bioethanol production

Indexes Strains 8.0% (v/v)
Initial ethanol
(YPD150)
44 °C
(YPD100)
43 °C + 2.6% (v/v)
Initial ethanol
(YPD100)
28% Glucose
(YPD280)
1.5 M NaCl
(YPD150)
Final generated ethanol concentration (g/L) E-158 28.64 ± 1.66bc 38.12 ± 1.90b 18.66 ± 1.62b 128.40 ± 1.17b 28.13 ± 1.58c
E-158-CRZ1 25.24 ± 1.99c 42.64 ± 1.71a 24.34 ± 1.76a 131.77 ± 0.79a 46.90 ± 2.10b
E-158-ENA5 32.39 ± 1.02a 43.52 ± 1.17a 23.43 ± 0.95a 132.90 ± 1.80a 56.73 ± 1.05a
  E-158 78.00 ± 3.40a 17.91 ± 1.72a 56.25 ± 3.00a 25.75 ± 2.22a 72.47 ± 4.53a
Residual glucose (g/L) E-158-CRZ1 82.34 ± 2.35a 9.70 ± 2.66b 43.99 ± 3.30b 19.21 ± 1.95b 34.24 ± 4.36b
E-158-ENA5 70.44 ± 1.74b 7.21 ± 2.03b 44.13 ± 1.40b 17.79 ± 2.79b 8.75 ± 2.54c
Ethanol yield
(g ethanol/g consumed glucose)
E-158 0.45 ± 0.01ab 0.46 ± 0.00a 0.44 ± 0.00a 0.50 ± 0.00a 0.40 ± 0.01b
E-158-CRZ1 0.43 ± 0.01b 0.47 ± 0.01a 0.44 ± 0.01a 0.50 ± 0.01a 0.43 ± 0.00a
E-158-ENA5 0.46 ± 0.01a 0.47 ± 0.01a 0.43 ± 0.01a 0.50 ± 0.01a 0.42 ± 0.01a
  1. The data in the table are those at the end of fermentation. Values indicate mean ± standard deviation of three biological replications. Values followed by different lowercase letters in the same column indicate significant differences at the level of P < 0.05 (Tukey-test) among strains. Same lowercase letters, no difference. E-158-CRZ1: Overexpression of TF Crz1p in E-158; E-158-ENA5: Overexpression of ENA5 in E-158