Table 5 Target sequences used in yeast transformation
Target | Sequence | |
|---|---|---|
gRNA insert | ||
tgR-F | TGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGA TAAATGATC | |
tgR-R | GTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGC TCTAAAAC | |
ENA5 | CS | GATAACGTATGTACTCACTGAGG |
CRZ1 | CS | GCAGAATGTCTACTACGTCGAGG |
ASP3 | CS | GCTACGGCATGGATCAGATTAGG |
TOS8 | CS | AGAAATTACATAATAACTGTAGG |
YOL162W | CS | AAGGTACAATGTTTAATAAATGG |
YOR012W | CS | TTGAAACTTTTTCAGTGATTGGG |
Repair fragment | ||
TEF1 | F | CACACACCATAGCTTCAAAATG |
R | TTTGTAATTAAAACT | |
ENA5 | RF F | CCTTCATCCTTTACATCGAGAATACGTTAACCAAATCAACCACACACCATAGCTTCAAAATG |
RF R | ATTCTTCATTATTGTTTTCTTTGACAGTTCCCTCGCTCATTTTGTAATTAAAACT | |
VP F | TTGTGAGGCTGATGTTTTCTTC | |
VP R | GCTTCTTCTGTAGTCAATGTGTGAT | |
CRZ1 | RF F | GGGCTGAAAAGTACATCCGCGCATTTAACAATTGCTAAGCCACACACCATAGCTTCAAAATG |
RF R | TAGTCATGTAGGAAGCCATATTTCCGTTGCTGAATGACATTTTGTAATTAAAACT | |
VP F | GCTTTGACTGCACTTTAGCTTAG | |
VP R | TTTCCGTTGCTGAATGACAT | |
ASP3 | RF F | AGAGCAAATGTTGGCTCGCTATTCTTTTGTAAGCAATCTGGTACTCACCAACCTCCAACTAGCCTGATCAGTGACTTTTCATCACACTGTGTTTTTATATAGTTCTTAGTAGTAAATATA |
RF R | TATATTTACTACTAAGAACTATATAAAAACACAGTGTGATGAAAAGTCACTGATCAGGCTAGTTGGAGGTTGGTGAGTACCAGATTGCTTACAAAAGAATAGCGAGCCAACATTTGCTCT | |
VP F | TATCAGACCCTTCAGCACGT | |
VP R | TGACACTGCTCAAGGGATAA | |
TOS8 | RF F | TTTTTCAGTATAGGAAGTAATCACTGTAGAAATAAGTCAACAATAATTGCATAGAAAAAATTTTACTTTTTTCGGAATTACCTAAAATGGGTTTACGGCATAGAAGATAGATAGATTAAG |
RF R | CTTAATCTATCTATCTTCTATGCCGTAAACCCATTTTAGGTAATTCCGAAAAAAGTAAAATTTTTTCTATGCAATTATTGTTGACTTATTTCTACAGTGATTACTTCCTATACTGAAAAA | |
VP F | GTTCCCTTGTTTTGAAGCAC | |
VP R | CGAAGATTCTCACCAAAGTT | |
YOL162W | RF F | ATGTCACTTAAAATGTTATGGCAGGGGATAACAGATTACTATATATAGCCTATCTACTTGACTATGTAGAAATATGGATACAATCTCCATGTTATGTATTTTTTAAGTTTGTGAATCATT |
RF R | AATGATTCACAAACTTAAAAAATACATAACATGGAGATTGTATCCATATTTCTACATAGTCAAGTAGATAGGCTATATATAGTAATCTGTTATCCCCTGCCATAACATTTTAAGTGACAT | |
VP F | CTAAGCAATCACCTAAACAT | |
VP R | GATGTCGTACTTCTACAGCT | |
YOR012W | RF F | GAAAAAGGCAGTGACAAAAATACTAATCAGAACGTTGAAAACAAATCAATAGTTTTGATACCATCCCGAAATTAGAGGTTCAGTCAGAAAAATACTCGAAAAATATAAAACCAAAGCAGA |
RF R | TCTGCTTTGGTTTTATATTTTTCGAGTATTTTTCTGACTGAACCTCTAATTTCGGGATGGTATCAAAACTATTGATTTGTTTTCAACGTTCTGATTAGTATTTTTGTCACTGCCTTTTTC | |
VP F | TGCCTCATAACGTCTTGGGG | |
VP R | GTAGGCCGTGAATCCCTTCC | |