Skip to main content

Table 1 Mass of lignin, cellulosic and non-cellulosic sugars in starting biomass and residual solids during CBP by C. thermocellum

From: Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum

Cell wall component Starting biomass (mg)a Final solid residue (mg) Amount solubilized by C. thermocellum CBP (mg) Percentage (%) solubilization by C. thermocellum CBP
Control CBP Gross Net Gross Net Netb
Cellulosic sugar
 Glc (SH) 2307 2191 1722 585 469 25 20 21
 Glc (MA) 2528 2394 1789 739 605 29 24 25
Non-cellulosic sugar
 Ara 54 49 42 12 7 23 13 15
 Rha 25 20 25 0 − 5 0 − 21 − 27
 Fuc 5 4 3 2 1 32 12 15
 Xyl 1249 1160 995 253 165 20 13 14
 GlcA 13 12 12 1 0 11 3 3
 GalA 107 83 24 82 58 77 55 71
 Man 65 61 39 26 22 40 34 36
 Gal 53 49 42 11 7 21 12 13
 Glc 158 148 114 44 34 28 21 23
 Total 1729 1584 1296 433 288 25 17 18
Lignin
 S lignin 733 525 584 149 − 59 20 − 8 − 11
 G lignin 321 260 270 51 − 10 16 − 3 − 4
 H lignin 13 11 12 1 − 1 8 − 8 − 9
 Total lignin 1323 1017 1105 218 − 88 16 − 7 − 9
  1. All data are mg per fermentation reaction, rounded to nearest integer. Zero and negative values are in bold. Gross—total solubilization of residual pellet in CPB fermenter. Net—solubilization of CPB fermenter residual pellet minus fermentation control residual pellet. Percentage solubilization is mass % of sugar in starting biomass
  2. SH saeman hydrolysis, MA methylated alditol
  3. aThe amount of starting biomass was 5.68 g
  4. bNet percent solubilization is mass % of net sugar in final residue of fermentation control