Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum

Table 1 Mass of lignin, cellulosic and non-cellulosic sugars in starting biomass and residual solids during CBP by C. thermocellum

Cell wall component	Starting biomass (mg)^a	Final solid residue (mg)		Amount solubilized by C. thermocellum CBP (mg)		Percentage (%) solubilization by C. thermocellum CBP
Cell wall component	Starting biomass (mg)^a	Control	CBP	Gross	Net	Gross	Net	Net^b
Cellulosic sugar
Glc (SH)	2307	2191	1722	585	469	25	20	21
Glc (MA)	2528	2394	1789	739	605	29	24	25
Non-cellulosic sugar
Ara	54	49	42	12	7	23	13	15
Rha	25	20	25	0	− 5	0	− 21	− 27
Fuc	5	4	3	2	1	32	12	15
Xyl	1249	1160	995	253	165	20	13	14
GlcA	13	12	12	1	0	11	3	3
GalA	107	83	24	82	58	77	55	71
Man	65	61	39	26	22	40	34	36
Gal	53	49	42	11	7	21	12	13
Glc	158	148	114	44	34	28	21	23
Total	1729	1584	1296	433	288	25	17	18
Lignin
S lignin	733	525	584	149	− 59	20	− 8	− 11
G lignin	321	260	270	51	− 10	16	− 3	− 4
H lignin	13	11	12	1	− 1	8	− 8	− 9
Total lignin	1323	1017	1105	218	− 88	16	− 7	− 9

All data are mg per fermentation reaction, rounded to nearest integer. Zero and negative values are in bold. Gross—total solubilization of residual pellet in CPB fermenter. Net—solubilization of CPB fermenter residual pellet minus fermentation control residual pellet. Percentage solubilization is mass % of sugar in starting biomass
SH saeman hydrolysis, MA methylated alditol
^aThe amount of starting biomass was 5.68 g
^bNet percent solubilization is mass % of net sugar in final residue of fermentation control

ISSN: 2731-3654