|
Cell wall component
|
Starting biomass (mg)a
|
Final solid residue (mg)
|
Amount solubilized by C. thermocellum CBP (mg)
|
Percentage (%) solubilization by C. thermocellum CBP
|
|---|
|
Control
|
CBP
|
Gross
|
Net
|
Gross
|
Net
|
Netb
|
|---|
|
Cellulosic sugar
|
|
Glc (SH)
|
2307
|
2191
|
1722
|
585
|
469
|
25
|
20
|
21
|
|
Glc (MA)
|
2528
|
2394
|
1789
|
739
|
605
|
29
|
24
|
25
|
|
Non-cellulosic sugar
|
|
Ara
|
54
|
49
|
42
|
12
|
7
|
23
|
13
|
15
|
|
Rha
|
25
|
20
|
25
|
0
|
− 5
|
0
|
− 21
|
− 27
|
|
Fuc
|
5
|
4
|
3
|
2
|
1
|
32
|
12
|
15
|
|
Xyl
|
1249
|
1160
|
995
|
253
|
165
|
20
|
13
|
14
|
|
GlcA
|
13
|
12
|
12
|
1
|
0
|
11
|
3
|
3
|
|
GalA
|
107
|
83
|
24
|
82
|
58
|
77
|
55
|
71
|
|
Man
|
65
|
61
|
39
|
26
|
22
|
40
|
34
|
36
|
|
Gal
|
53
|
49
|
42
|
11
|
7
|
21
|
12
|
13
|
|
Glc
|
158
|
148
|
114
|
44
|
34
|
28
|
21
|
23
|
|
Total
|
1729
|
1584
|
1296
|
433
|
288
|
25
|
17
|
18
|
|
Lignin
|
|
S lignin
|
733
|
525
|
584
|
149
|
− 59
|
20
|
− 8
|
− 11
|
|
G lignin
|
321
|
260
|
270
|
51
|
− 10
|
16
|
− 3
|
− 4
|
|
H lignin
|
13
|
11
|
12
|
1
|
− 1
|
8
|
− 8
|
− 9
|
|
Total lignin
|
1323
|
1017
|
1105
|
218
|
− 88
|
16
|
− 7
|
− 9
|
- All data are mg per fermentation reaction, rounded to nearest integer. Zero and negative values are in bold. Gross—total solubilization of residual pellet in CPB fermenter. Net—solubilization of CPB fermenter residual pellet minus fermentation control residual pellet. Percentage solubilization is mass % of sugar in starting biomass
- SH saeman hydrolysis, MA methylated alditol
- aThe amount of starting biomass was 5.68 g
- bNet percent solubilization is mass % of net sugar in final residue of fermentation control
