Fig. 5

Characterization of FAD2-like protein in V. galamensis. a Phylogenetic comparison of VgFAD2-like, VgFAD2 and other selected plant FAD2 and divergent forms of FAD2 sequences. The tree was generated by ClustalW, Neighbor-Joining (NJ) method was adopted. Sequences used to construct the tree included Arabidopsis thaliana oleate desaturase AtFAD2 (NM_112047), Ricinus communis hydroxygenase RcFAH12 (U22378), Vernicia fordii conjugase VfFADX (AF525535), Crepis palaestina epoxygenase Cpal2 (Y16283), Stokesia laevis epoxygenase SlEPX (AY462108), Crepis alpine acetylenase Crepl (Y16285.2), Vernonia galamensis VgFAD2 (AF188264), Glycine max GmFAD2 (NP_001347010.1), Olea europaea OeFAD2 (QKI80086.1), Camellia oleifera CoFAD2 (QID89773.1), Brassica nigra BniFAD2 (ADJ58018.1), Brassica rapa BraFAD2 (XP_009146642.2), Brassica juncea BjuFAD2 (QGW48100.1), Camelina sativa CsaFAD2 (ADN10825.1), Jatropha curcas JcFAD2 (AEW43691.1), Vitis vinifera VvFAD2 (RVX12826.1), Helianthus annuus HaFAD2 (XP_035830434.1), Perilla frutescens PfFAD2 (APQ41587.1), Sesamum indicum SiFAD2 (XP_011075145.1) and Solanum tuberosum StFAD2 (XP_006365798.1). b The position of three conserved histidine boxes in FAD2-like protein sequence