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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Rational and evolutionary engineering of Saccharomyces cerevisiae for production of dicarboxylic acids from lignocellulosic biomass and exploring genetic mechanisms of the yeast tolerance to the biomass hydrolysate

Fig. 5

Production of dicarboxylic acids from xylose. a Schematic illustration of the metabolic pathways, where dicarboxylic acids are involved. Genes overexpressed in the evolved engineered strain are displayed. Engineered parts of the production pathway are highlighted by thick black arrows and heterologous genes displayed in green letters. b Dicarboxylic (malic, succinic, fumaric) acid production by XylC2 EV6_4 and EV6_4 MA strains in YEX medium with CaCO3 or c in YEX with no external buffer. Concentration of dicarboxylic acids is plotted on the secondary y-axis. The experiments were performed in duplicates, error bars represent standard deviation (N = 2)

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