Fig. 3
From: PACER: a novel 3D plant cell wall model for the analysis of non-catalytic and enzymatic responses
Impact of loosenin-like proteins on NpXyn11A migration. Composites were pretreated with 0.1 mg/mL PcaLOOL2, PcaLOOL12, BSA or buffer only at pH 5.0 and room temperature for 24 h. This was followed by incubation in 0.005 mg/mL NpXyn11A or buffer only, at pH 5.0, 40 °C and 300 rpm for 3–6 h. A Representative cellulose/azo-xylan composites dried after treatment. B Average intensity values of dried cellulose/azo-xylan composites per segment. C Absorbance of the reaction solution at 590 nm recovered after composite incubation; statistically significant differences compared to pretreatment with BSA are indicated by an asterisk (two-tailed t-test; p ≤ 0.05). For all experiments, n ≥ 4; error bars correspond to standard deviation of mean