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Table 2 Kinetic parameters 2-ketoisovalerate and pyruvate decarboxylation catalyzed by Kp-IpdC or KivD

From: The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae

Substrate

Enzyme

Km (mM)

Vmax (mM/min)

Kcat (s−1)

Kcat/Km (M−1 s−1)

2-Ketoisovalerate

Kp-IpdC

1.48 (1.20, 1.75)

0.037 (0.027, 0.056)

8.05

5445.39

KivD

4.18 (4.08, 4.23)

0.0409 (0.038, 0.044)

3.46

828.31

Pyruvate

Kp-IpdC

3.13 (2.91, 3.30)

0.0115 (0.0105, 0.0125)

0.58

183.70

KivD

18.81 (17.15, 20.85)

0.0034 (0.0029, 0.0039)

0.28

15.09

  1. 95% asymptotic confidence interval of Km and Vmax were given in brackets
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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