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Table 2 Kinetic parameters 2-ketoisovalerate and pyruvate decarboxylation catalyzed by Kp-IpdC or KivD

From: The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae

Substrate Enzyme Km (mM) Vmax (mM/min) Kcat (s−1) Kcat/Km (M−1 s−1)
2-Ketoisovalerate Kp-IpdC 1.48 (1.20, 1.75) 0.037 (0.027, 0.056) 8.05 5445.39
KivD 4.18 (4.08, 4.23) 0.0409 (0.038, 0.044) 3.46 828.31
Pyruvate Kp-IpdC 3.13 (2.91, 3.30) 0.0115 (0.0105, 0.0125) 0.58 183.70
KivD 18.81 (17.15, 20.85) 0.0034 (0.0029, 0.0039) 0.28 15.09
  1. 95% asymptotic confidence interval of Km and Vmax were given in brackets