Table 3 Kinetic parameters of variants of Kp-IpdC with 2-ketoisovalerate or pyruvate as substrates

Kp-IpdC

2-Ketoisovalerate

1.48 (1.20, 1.75)

0.037 (0.027, 0.056)

8.05

5445.39

Pyruvate

3.13 (2.91, 3.30)

0.0115 (0.0105, 0.0125)

0.58

183.70

A387L

2-Ketoisovalerate

5.91 (3.69, 7.69)

0.0024 (0.0013, 0.0089)

0.31

52.57

Pyruvate

13.19 (10.14, 16.38)

0.0024 (0.0020, 0.0031)

0.21

15.63

F388W

2-Ketoisovalerate

15.85 (15.22, 16.49)

0.019 (0.015, 0.024)

2.9

172.68

Pyruvate

n.d

n.d

n.d

n.d

V542I

2-Ketoisovalerate

1.99 (1.57, 2.40)

0.018 (0.015, 0.020)

2.88

1443.98

Pyruvate

23.38 (22.22, 24.82)

0.0056 (0.0048, 0.0067)

0.9

38.39

L546W

2-Ketoisovalerate

1.01 (0.96, 1.18)

0.034 (0.030, 0.038)

2.59

2559.61

Pyruvate

693.2 (677.3, 888.9)

0.085 (0, 0.096)

6.66

9.61

D289L + T290L

2-Ketoisovalerate

n.d

n.d

n.d

n.d

Pyruvate

n.d

n.d

n.d

n.d

A387I + F388W

2-Ketoisovalerate

48.07 (43.02, 52.33)

0.017 (0.006, 0.023)

0.704

14.653

Pyruvate

n.d

n.d

n.d

n.d

D289L

2-Ketoisovalerate

n.d

n.d

n.d

n.d

Pyruvate

n.d

n.d

n.d

n.d

T290L

2-Ketoisovalerate

0.98 (0.91, 1.05)

0.038 (0.036, 0.041)

2.97

2609.8

Pyruvate

13.01 (9.55, 16.46)

0.0057 (0.0039, 0.001)

0.46

40.5

Q383M

2-Ketoisovalerate

1.88 (0.177, 0.199)

0.030 (0.027, 0.034)

4.18

2215.2

Pyruvate

19.7 (18.1, 20.5)

0.016 (0.012, 0.020)

2.16

109.4

A387I

2-Ketoisovalerate

23.4 (22.9, 23.9)

0.034 (0.029, 0.041)

1.61

68.62

Pyruvate

39.7 (36.7, 43.3)

0.0051 (0.0036, 0.009)

0.24

6.02