|
Variants
|
Substrate
|
Km (mM)
|
Vmax (mM/min)
|
Kcat (s−1)
|
Kcat/Km (M−1 s−1)
|
|---|
|
Kp-IpdC
|
2-Ketoisovalerate
|
1.48 (1.20, 1.75)
|
0.037 (0.027, 0.056)
|
8.05
|
5445.39
|
|
Pyruvate
|
3.13 (2.91, 3.30)
|
0.0115 (0.0105, 0.0125)
|
0.58
|
183.70
|
|
A387L
|
2-Ketoisovalerate
|
5.91 (3.69, 7.69)
|
0.0024 (0.0013, 0.0089)
|
0.31
|
52.57
|
|
Pyruvate
|
13.19 (10.14, 16.38)
|
0.0024 (0.0020, 0.0031)
|
0.21
|
15.63
|
|
F388W
|
2-Ketoisovalerate
|
15.85 (15.22, 16.49)
|
0.019 (0.015, 0.024)
|
2.9
|
172.68
|
|
Pyruvate
|
n.d
|
n.d
|
n.d
|
n.d
|
|
V542I
|
2-Ketoisovalerate
|
1.99 (1.57, 2.40)
|
0.018 (0.015, 0.020)
|
2.88
|
1443.98
|
|
Pyruvate
|
23.38 (22.22, 24.82)
|
0.0056 (0.0048, 0.0067)
|
0.9
|
38.39
|
|
L546W
|
2-Ketoisovalerate
|
1.01 (0.96, 1.18)
|
0.034 (0.030, 0.038)
|
2.59
|
2559.61
|
|
Pyruvate
|
693.2 (677.3, 888.9)
|
0.085 (0, 0.096)
|
6.66
|
9.61
|
|
D289L + T290L
|
2-Ketoisovalerate
|
n.d
|
n.d
|
n.d
|
n.d
|
|
Pyruvate
|
n.d
|
n.d
|
n.d
|
n.d
|
|
A387I + F388W
|
2-Ketoisovalerate
|
48.07 (43.02, 52.33)
|
0.017 (0.006, 0.023)
|
0.704
|
14.653
|
|
Pyruvate
|
n.d
|
n.d
|
n.d
|
n.d
|
|
D289L
|
2-Ketoisovalerate
|
n.d
|
n.d
|
n.d
|
n.d
|
|
Pyruvate
|
n.d
|
n.d
|
n.d
|
n.d
|
|
T290L
|
2-Ketoisovalerate
|
0.98 (0.91, 1.05)
|
0.038 (0.036, 0.041)
|
2.97
|
2609.8
|
|
Pyruvate
|
13.01 (9.55, 16.46)
|
0.0057 (0.0039, 0.001)
|
0.46
|
40.5
|
|
Q383M
|
2-Ketoisovalerate
|
1.88 (0.177, 0.199)
|
0.030 (0.027, 0.034)
|
4.18
|
2215.2
|
|
Pyruvate
|
19.7 (18.1, 20.5)
|
0.016 (0.012, 0.020)
|
2.16
|
109.4
|
|
A387I
|
2-Ketoisovalerate
|
23.4 (22.9, 23.9)
|
0.034 (0.029, 0.041)
|
1.61
|
68.62
|
|
Pyruvate
|
39.7 (36.7, 43.3)
|
0.0051 (0.0036, 0.009)
|
0.24
|
6.02
|
- 95% asymptotic confidence interval of Km and Vmax were given in brackets
- nd not detectable
