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Table 3 Kinetic parameters of variants of Kp-IpdC with 2-ketoisovalerate or pyruvate as substrates

From: The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae

Variants Substrate Km (mM) Vmax (mM/min) Kcat (s−1) Kcat/Km (M−1 s−1)
Kp-IpdC 2-Ketoisovalerate 1.48 (1.20, 1.75) 0.037 (0.027, 0.056) 8.05 5445.39
Pyruvate 3.13 (2.91, 3.30) 0.0115 (0.0105, 0.0125) 0.58 183.70
A387L 2-Ketoisovalerate 5.91 (3.69, 7.69) 0.0024 (0.0013, 0.0089) 0.31 52.57
Pyruvate 13.19 (10.14, 16.38) 0.0024 (0.0020, 0.0031) 0.21 15.63
F388W 2-Ketoisovalerate 15.85 (15.22, 16.49) 0.019 (0.015, 0.024) 2.9 172.68
Pyruvate n.d n.d n.d n.d
V542I 2-Ketoisovalerate 1.99 (1.57, 2.40) 0.018 (0.015, 0.020) 2.88 1443.98
Pyruvate 23.38 (22.22, 24.82) 0.0056 (0.0048, 0.0067) 0.9 38.39
L546W 2-Ketoisovalerate 1.01 (0.96, 1.18) 0.034 (0.030, 0.038) 2.59 2559.61
Pyruvate 693.2 (677.3, 888.9) 0.085 (0, 0.096) 6.66 9.61
D289L + T290L 2-Ketoisovalerate n.d n.d n.d n.d
Pyruvate n.d n.d n.d n.d
A387I + F388W 2-Ketoisovalerate 48.07 (43.02, 52.33) 0.017 (0.006, 0.023) 0.704 14.653
Pyruvate n.d n.d n.d n.d
D289L 2-Ketoisovalerate n.d n.d n.d n.d
Pyruvate n.d n.d n.d n.d
T290L 2-Ketoisovalerate 0.98 (0.91, 1.05) 0.038 (0.036, 0.041) 2.97 2609.8
Pyruvate 13.01 (9.55, 16.46) 0.0057 (0.0039, 0.001) 0.46 40.5
Q383M 2-Ketoisovalerate 1.88 (0.177, 0.199) 0.030 (0.027, 0.034) 4.18 2215.2
Pyruvate 19.7 (18.1, 20.5) 0.016 (0.012, 0.020) 2.16 109.4
A387I 2-Ketoisovalerate 23.4 (22.9, 23.9) 0.034 (0.029, 0.041) 1.61 68.62
Pyruvate 39.7 (36.7, 43.3) 0.0051 (0.0036, 0.009) 0.24 6.02
  1. 95% asymptotic confidence interval of Km and Vmax were given in brackets
  2. nd not detectable