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Table 2 Growth characteristics of IMZ785 and IMS1215 in sucrose-limited anaerobic steady-state chemostat cultures at a dilution rate of 0.1 h−1

From: Engineering proton-coupled hexose uptake in Saccharomyces cerevisiae for improved ethanol yield

Strain IMZ785 IMS1215
Relevant genotype HXT5 HGT1 + FSY1
Carbon source Sucrose Sucrose
µmax (h−1a 0.28 ± 0.00 0.15 ± 0.00
Biomass yield (gx ghexose eq −1) 0.088 ± 0.005 0.047 ± 0.000
Ethanol yield (mol molhexose eq−1) 1.51 ± 0.00 1.76 ± 0.02
qs (mmol gx−1 h−1) 6.41 ± 0.43 11.9 ± 0.23
qethanol (mmol gx−1 h−1) 9.71 ± 0.63 20.9 ± 0.19
qglycerol (mmol gx−1 h−1) 0.86 ± 0.08 0.85 ± 0.02
qCO2 (mmol gx−1 h−1) 10.1 ± 0.76 20.2 ± 0.40
Residual sugar (g L−1) 0.40 ± 0.01 (glucose)
0.56 ± 0.07 (fructose)
0.70 ± 0.00 (glucose)
4.86 ± 0.10 (fructose)
Carbon recovery (%) b 95.8 ± 0.10 97.7 ± 0.45
Actual dilution rate (h−1) 0.102 ± 0.001 0.102 ± 0.002
  1. aThe maximum specific growth rate (µmax) was determined based on measurements of the CO2 concentration in the reactor off-gas during the preceding batch phase
  2. Biomass-specific production and consumption rates are depicted as qmetabolite
  3. bThe carbon recovery represents the percentage of the carbon entering the reactor system via the medium that could be traced back in biomass, products and residual substrate
  4. The data represent average values and mean deviations obtained from duplicate experiments