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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion

Fig. 3

Engineering for simultaneous utilization of lignocellulose-derived sugars. A Altered PTS to enable co-consumption of glucose, xylose, and arabinose. In the wild type, CRP cannot activate genes for arabinose and xylose utilization. Inactivation of PtsG increases the concentration of phosphorylated EIIA, which activates AC and increases cAMP concentration. CRP activated by cAMP promotes the transcription of genes related to the catabolism of xylose and arabinose. Glucose can be transported through GalP instead of EIIBC. Abbreviations: Glc, glucose; Xyl, xylose; Ara, arabinose; G6P, glucose-6-phosphate; PEP, phosphoenolpyruvate; PYR, pyruvate; EI, enzyme I; HPr, histidine protein; EIIA, enzyme II A component; EIIBC, enzyme II BC component; AC, adenylate cyclase; ATP, adenosine triphosphate; cAMP, cyclic adenosine monophosphate; CRP, cAMP receptor protein; GalP, galactose–proton symporter. Growth profiles of B wild-type Vibrio sp. dhg, C VXA38-1, D VXA38P, and E VXA38PG strains in minimal medium containing 2 g/L of glucose, xylose, and arabinose. The left and right y-axes indicate cell biomass (OD600) and sugar concentration (g/L), respectively, and the x-axis indicates time (h). Symbols: black circles, OD600; blue squares, glucose; green diamonds, xylose; purple triangles, arabinose; yellow inverted triangle, acetate

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