Fig. 5

Effect of dockerin addition on the activity of T. fusca mannosidase on different substrates. The activity levels of Tf-Manno (construct nr. 32) and Tf-Manno_Doc-Bc (construct nr. 39) are shown. A. Activity analysis on pNP-β-mannose. Reaction mixtures were composed of 1 mL 2.5 mM pNP-β-mannose and 2 pmol enzyme. Enzymatic activity was determined quantitatively by measuring the released pNP. B. Activity analysis on mannan. Reaction mixtures were composed of 1 mL 0.1% mannan and 48 pmol enzyme. Enzymatic activity was determined quantitatively by measuring the released reducing sugars with the DNS method. C. Activity analysis on GM. Reaction mixtures were composed of 1 mL 0.5% GM and 96 pmol enzyme. Enzymatic activity was determined quantitatively by measuring the released reducing sugars with the DNS method. All slopes were converted to enzymatic activity (U/nmol). Each bar represents the mean ± SD of three replicates. *p < 0.05, Student’s t test