Fig. 8
From: Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study
Influence of type of CBM module in the scaffoldin on the activity of the cellulosome complex. The activity of the cellulosome complex composed of Ct-CBM3_Coh-Ac_Coh-Bc_Coh-CtI (construct nr. 135), Tf-Manna_Li-G_Doc-Ac (construct nr. 26), Tf-Manno_Doc-Bc (construct nr. 39) and Cj-Aga_Li-D_Doc-CtI (construct nr. 63) and the complex composed of Cj-CBM35_Coh-Ac_Coh-Bc_Coh-CtI (construct nr. 134), Tf-Manna_Li-G_Doc-Ac (construct nr. 26), Tf-Manno_Doc-Bc (construct nr. 39) and Cj-Aga_Li-D_Doc-CtI (construct nr. 63) was tested. Reaction mixtures were composed of 1 mL 0.5% GM and 10 pmol designer cellulosome. Enzymatic activity was determined quantitatively by measuring the released reducing sugars with the DNS method. Each bar represents the mean ± SD of three independent experiments. The Student’s t-test revealed that there was no significant difference in activity between the two types of designer cellulosomes (p = 0.381)