Table 1 C. tropicalis strains used in this study
From: Engineering the oleaginous yeast Candida tropicalis for α-humulene overproduction
Strains | Genotypes | References |
|---|---|---|
C. tropicalis ATCC 20,336 | URA3/URA3, CAT/CAT, FAO1/FAO, ALD1/ALD1, POX5/POX5, DLD1a/DLD1a, DLD1b/DLD1b, LPP2/LPP2, ERG9/ERG9 | ATCC |
C. tropicalis CU-206 | C. tropicalis ATCC 20,336, ura3/ura3 | [25] |
C. tropicalis 05-3 | C. tropicalis ATCC 20,336, ura3/ura3, cat::gda324/cat::gda324-URA3-PGAP1-yeGFP3-TGAP1 | [31] |
C. tropicalis 01 | C. tropicalis CU-206, CAT/cat::gda-PGAP1-ZSS1-yeGFP3-PTS1-TGAP1, ALD1/ald1::gda-URA3-PGAP1-PEX3-mScarlet-TGAP1 | This study |
C. tropicalis 02 | C. tropicalis CU-206, CAT/cat::gda-URA3-PGAP1-yeGFP3-Tsynth7 | This study |
CU-207 | C. tropicalis CU-206, CAT/cat::gda-URA3-PGAP1-Cas9-3 × SV40-TENO1 | This study |
CU-208 | CU-207, CAT/cat::gda-PGAP1-Cas9-3 × SV40-TENO1 | This study |
HC01 | CU-208, POX5/pox5::gda-URA3-PFBA1-ZSS1-TADH2 | This study |
HC02 | CU-208, pox5::gda-URA3-PFBA1-ZSS1-TADH2/pox5::gda-URA3-PFBA1-ZSS1-TADH2 | This study |
HC03 | CU-208, pox5::gda-PFBA1-ZSS1-TADH2/pox5::gda-PFBA1-ZSS1-TADH2 | This study |
HP01 | CU-208, POX5/pox5::gda-URA3-PFBA1-ZSS1-PTS1-TADH2 | This study |
HP02 | CU-208, pox5::gda-URA3-PFBA1-ZSS1-PTS1-TADH2/pox5::gda-URA3-PFBA1-ZSS1-PTS1-TADH2 | This study |
HC04 | HC03, fao1::gda-URA3-PGAP1-ERG10-TENO1/fao1::gda-URA3-PGAP1-ERG10-TENO1 | This study |
HC05 | HC03, fao1::gda-URA3-PGAP1-HMGR-Tsynth7/fao1::gda-URA3-PGAP1-HMGR-Tsynth7 | This study |
HC06 | HC03, fao1::gda-URA3-PGAP1-tHMGR-Tsynth7/fao1::gda-URA3-PGAP1-tHMGR-Tsynth7 | This study |
HC07 | HC03, ERG9/erg9::gda-URA3 | This study |
SC-H01 | CU-208, FAO1/fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1, ALD1/ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1, POX5/pox5::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 | This study |
DC-H01 | CU-208, fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1/fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1, ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1/ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1, pox5::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1/pox5::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 | This study |
DC-H02 | CU-208, fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1/fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1, ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1/ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1, pox5::gda-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1/pox5::gda-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 | This study |
SP-H01 | CU-208, FAO1/fao1::gda-TPGK1-PTS1-ERG13-PFBA1-PGAP1-tHMGR-PTS1-Tsynth7-PGAP1-ERG10-PTS1-TENO1, ALD1/ald1::gda-TPGK1-PTS1-ERG12-PFBA1-PGAP1-ERG8-PTS1-Tsynth7-TADH2-PTS1-ERG19-PFBA1-PGAP1-IDI1-PTS1-TENO1, POX5/pox5::gda-URA3-PGAP1-ERG20-PTS1-Tsynth7-TADH2-PTS1-ZSS1-PFBA1 | This study |
DP-H01 | CU-208, fao1::gda-TPGK1-PTS1-ERG13-PFBA1-PGAP1-tHMGR-PTS1-Tsynth7-PGAP1-ERG10-PTS1-TENO1/fao1::gda-TPGK1-PTS1-ERG13-PFBA1-PGAP1-tHMGR-PTS1-Tsynth7-PGAP1-ERG10-PTS1-TENO1, ald1::gda-TPGK1-PTS1-ERG12-PFBA1-PGAP1-ERG8-PTS1-Tsynth7-TADH2-PTS1-ERG19-PFBA1-PGAP1-IDI1-PTS1-TENO1/ald1::gda-TPGK1-PTS1-ERG12-PFBA1-PGAP1-ERG8-PTS1-Tsynth7-TADH2-PTS1-ERG19-PFBA1-PGAP1-IDI1-PTS1-TENO1, pox5::gda-URA3-PGAP1-ERG20-PTS1-Tsynth7-TADH2-PTS1-ZSS1-PFBA1/pox5::gda-URA3-PGAP1-ERG20-PTS1-Tsynth7-TADH2-PTS1-ZSS1-PFBA1 | This study |
DC-H03S | DC-H02, DLD1a/dld1a::gda-URA3-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1 | This study |
DC-H03D | DC-H02, dld1a::gda-URA3-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1/dld1a::gda-URA3-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1 | This study |
DC-H05S | DC-H02, DLD1a/dld1a::gda-URA3-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1 | This study |
DC-H05D | DC-H02, dld1a::gda-URA3-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1/dld1a::gda-URA3-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1 | This study |
DC-H07S | DC-H02, DLD1a/dld1a::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 | This study |
DC-H07D | DC-H02, dld1a::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1/dld1a::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 | This study |
DC-H08 | DC-H02, dld1a::gda-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1/dld1a::gda-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 | This study |
DC-H09D | DC-H02, dld1a::gda-URA3-TADH2-ZSS1-PFBA1/dld1a::gda-URA3-TADH2-ZSS1-PFBA1 | this study |
DC-H11S | DC-H08, LPP2/lpp2::gda-URA3-PGAP1-ZSS1-Tsynth7 | This study |
DC-H11D | DC-H08, lpp2::gda-URA3-PGAP1-ZSS1-Tsynth7/lpp2::gda-URA3-PGAP1-ZSS1-Tsynth7 | This study |
DC-H12 | DC-H08, lpp2::gda-PGAP1-ZSS1-Tsynth7/lpp2::gda-PGAP1-ZSS1-Tsynth7 | This study |
DC-H13S | DC-H12, DLD1b/dld1b::gda-URA3-PGAP1-ZSS1-Tsynth7 | This study |
DC-H13D | DC-H12, dld1b::gda-URA3-PGAP1-ZSS1-Tsynth7/dld1b::gda-URA3-PGAP1-ZSS1-Tsynth7 | This study |
DC-H14 | DC-H12, dld1b::gda-PGAP1-ZSS1-Tsynth7/dld1b::gda-PGAP1-ZSS1-Tsynth7 | This study |
DC-H15D | DC-H08, lpp2::gda-URA3/lpp2::gda-URA3 | This study |
DC-H17D | DC-H12, dld1b::gda-URA3-PFBA1-tHMGR-TADH2-Tsynth7-ZSS1-PGAP1/dld1b::gda-URA3-PFBA1-tHMGR-TADH2-Tsynth7-ZSS1-PGAP1 | This study |
DC-H19D | DC-H12, dld1b::gda-URA3-PFBA1-NADH-HMGR-TADH2-Tsynth7-ZSS1-PGAP1/dld1b::gda-URA3-PFBA1-NADH-HMGR-TADH2-Tsynth7-ZSS1-PGAP1 | This study |
DC-H21D | DC-H12, dld1b::gda-URA3-TPGK1-NADH-HMGR-PFBA1-PGAP1-ZSS1-Tsynth7-PGAP1-ERG10-TENO1/ dld1b::gda-URA3-TPGK1-NADH-HMGR-PFBA1-PGAP1-ZSS1-Tsynth7-PGAP1-ERG10-TENO1 | This study |