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Table 1 C. tropicalis strains used in this study

From: Engineering the oleaginous yeast Candida tropicalis for α-humulene overproduction

Strains Genotypes References
C. tropicalis ATCC 20,336 URA3/URA3, CAT/CAT, FAO1/FAO, ALD1/ALD1, POX5/POX5, DLD1a/DLD1a, DLD1b/DLD1b, LPP2/LPP2, ERG9/ERG9 ATCC
C. tropicalis CU-206 C. tropicalis ATCC 20,336, ura3/ura3 [25]
C. tropicalis 05-3 C. tropicalis ATCC 20,336, ura3/ura3, cat::gda324/cat::gda324-URA3-PGAP1-yeGFP3-TGAP1 [31]
C. tropicalis 01 C. tropicalis CU-206, CAT/cat::gda-PGAP1-ZSS1-yeGFP3-PTS1-TGAP1, ALD1/ald1::gda-URA3-PGAP1-PEX3-mScarlet-TGAP1 This study
C. tropicalis 02 C. tropicalis CU-206, CAT/cat::gda-URA3-PGAP1-yeGFP3-Tsynth7 This study
CU-207 C. tropicalis CU-206, CAT/cat::gda-URA3-PGAP1-Cas9-3 × SV40-TENO1 This study
CU-208 CU-207, CAT/cat::gda-PGAP1-Cas9-3 × SV40-TENO1 This study
HC01 CU-208, POX5/pox5::gda-URA3-PFBA1-ZSS1-TADH2 This study
HC02 CU-208, pox5::gda-URA3-PFBA1-ZSS1-TADH2/pox5::gda-URA3-PFBA1-ZSS1-TADH2 This study
HC03 CU-208, pox5::gda-PFBA1-ZSS1-TADH2/pox5::gda-PFBA1-ZSS1-TADH2 This study
HP01 CU-208, POX5/pox5::gda-URA3-PFBA1-ZSS1-PTS1-TADH2 This study
HP02 CU-208, pox5::gda-URA3-PFBA1-ZSS1-PTS1-TADH2/pox5::gda-URA3-PFBA1-ZSS1-PTS1-TADH2 This study
HC04 HC03, fao1::gda-URA3-PGAP1-ERG10-TENO1/fao1::gda-URA3-PGAP1-ERG10-TENO1 This study
HC05 HC03, fao1::gda-URA3-PGAP1-HMGR-Tsynth7/fao1::gda-URA3-PGAP1-HMGR-Tsynth7 This study
HC06 HC03, fao1::gda-URA3-PGAP1-tHMGR-Tsynth7/fao1::gda-URA3-PGAP1-tHMGR-Tsynth7 This study
HC07 HC03, ERG9/erg9::gda-URA3 This study
SC-H01 CU-208, FAO1/fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1, ALD1/ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1, POX5/pox5::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 This study
DC-H01 CU-208, fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1/fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1, ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1/ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1, pox5::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1/pox5::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 This study
DC-H02 CU-208, fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1/fao1::gda-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1, ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1/ald1::gda-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1, pox5::gda-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1/pox5::gda-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 This study
SP-H01 CU-208, FAO1/fao1::gda-TPGK1-PTS1-ERG13-PFBA1-PGAP1-tHMGR-PTS1-Tsynth7-PGAP1-ERG10-PTS1-TENO1, ALD1/ald1::gda-TPGK1-PTS1-ERG12-PFBA1-PGAP1-ERG8-PTS1-Tsynth7-TADH2-PTS1-ERG19-PFBA1-PGAP1-IDI1-PTS1-TENO1, POX5/pox5::gda-URA3-PGAP1-ERG20-PTS1-Tsynth7-TADH2-PTS1-ZSS1-PFBA1 This study
DP-H01 CU-208, fao1::gda-TPGK1-PTS1-ERG13-PFBA1-PGAP1-tHMGR-PTS1-Tsynth7-PGAP1-ERG10-PTS1-TENO1/fao1::gda-TPGK1-PTS1-ERG13-PFBA1-PGAP1-tHMGR-PTS1-Tsynth7-PGAP1-ERG10-PTS1-TENO1, ald1::gda-TPGK1-PTS1-ERG12-PFBA1-PGAP1-ERG8-PTS1-Tsynth7-TADH2-PTS1-ERG19-PFBA1-PGAP1-IDI1-PTS1-TENO1/ald1::gda-TPGK1-PTS1-ERG12-PFBA1-PGAP1-ERG8-PTS1-Tsynth7-TADH2-PTS1-ERG19-PFBA1-PGAP1-IDI1-PTS1-TENO1, pox5::gda-URA3-PGAP1-ERG20-PTS1-Tsynth7-TADH2-PTS1-ZSS1-PFBA1/pox5::gda-URA3-PGAP1-ERG20-PTS1-Tsynth7-TADH2-PTS1-ZSS1-PFBA1 This study
DC-H03S DC-H02, DLD1a/dld1a::gda-URA3-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1 This study
DC-H03D DC-H02, dld1a::gda-URA3-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1/dld1a::gda-URA3-TPGK1-ERG13-PFBA1-PGAP1-tHMGR-Tsynth7-PGAP1-ERG10-TENO1 This study
DC-H05S DC-H02, DLD1a/dld1a::gda-URA3-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1 This study
DC-H05D DC-H02, dld1a::gda-URA3-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1/dld1a::gda-URA3-TPGK1-ERG12-PFBA1-PGAP1-ERG8-Tsynth7-TADH2-ERG19-PFBA1-PGAP1-IDI1-TENO1 This study
DC-H07S DC-H02, DLD1a/dld1a::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 This study
DC-H07D DC-H02, dld1a::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1/dld1a::gda-URA3-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 This study
DC-H08 DC-H02, dld1a::gda-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1/dld1a::gda-PGAP1-ERG20-Tsynth7-TADH2-ZSS1-PFBA1 This study
DC-H09D DC-H02, dld1a::gda-URA3-TADH2-ZSS1-PFBA1/dld1a::gda-URA3-TADH2-ZSS1-PFBA1 this study
DC-H11S DC-H08, LPP2/lpp2::gda-URA3-PGAP1-ZSS1-Tsynth7 This study
DC-H11D DC-H08, lpp2::gda-URA3-PGAP1-ZSS1-Tsynth7/lpp2::gda-URA3-PGAP1-ZSS1-Tsynth7 This study
DC-H12 DC-H08, lpp2::gda-PGAP1-ZSS1-Tsynth7/lpp2::gda-PGAP1-ZSS1-Tsynth7 This study
DC-H13S DC-H12, DLD1b/dld1b::gda-URA3-PGAP1-ZSS1-Tsynth7 This study
DC-H13D DC-H12, dld1b::gda-URA3-PGAP1-ZSS1-Tsynth7/dld1b::gda-URA3-PGAP1-ZSS1-Tsynth7 This study
DC-H14 DC-H12, dld1b::gda-PGAP1-ZSS1-Tsynth7/dld1b::gda-PGAP1-ZSS1-Tsynth7 This study
DC-H15D DC-H08, lpp2::gda-URA3/lpp2::gda-URA3 This study
DC-H17D DC-H12, dld1b::gda-URA3-PFBA1-tHMGR-TADH2-Tsynth7-ZSS1-PGAP1/dld1b::gda-URA3-PFBA1-tHMGR-TADH2-Tsynth7-ZSS1-PGAP1 This study
DC-H19D DC-H12, dld1b::gda-URA3-PFBA1-NADH-HMGR-TADH2-Tsynth7-ZSS1-PGAP1/dld1b::gda-URA3-PFBA1-NADH-HMGR-TADH2-Tsynth7-ZSS1-PGAP1 This study
DC-H21D DC-H12, dld1b::gda-URA3-TPGK1-NADH-HMGR-PFBA1-PGAP1-ZSS1-Tsynth7-PGAP1-ERG10-TENO1/ dld1b::gda-URA3-TPGK1-NADH-HMGR-PFBA1-PGAP1-ZSS1-Tsynth7-PGAP1-ERG10-TENO1 This study