Fig. 1

Analysis of the effect of quenching ROS on cultivated cyanobacterial cell growth by adding ascorbic acid. Cell growth curves of Synechococcus cscB⁺ (A), H₂O₂ content (B) and ROS level (C) in the coculture system and axenic culture with additional ascorbic acid. The coculture system were cultivated in coculture medium (named CoBG-11) under a light intensity of approximately 100 μmol photons m⁻² s⁻¹ in an illuminating shaking incubator at 150 rpm and 30 °C. CoBG-11 medium was designed based on BG-11 medium and optimized for E. coli growth by supplementing with 150 mM NaCl, 4 mM NH₄Cl and 3 g/L 2-[[1,3-dihydroxy-2-(hydroxymethyl) propan-2-yl] amino] ethanesulfonic acid (TES). NH₄Cl were used to maintain the cell survival of E. coli, and NaCl was used as a stress inducer for sucrose accumulation in Synechococcus 2973