Fig. 4

Biochemical characterization of Cel776Ec and Cel776Sc. All measurements were the result of three independent experiments and an enzyme blank was subtracted to each condition tested. A Optimal temperature at pH 5. B Optimal pH at temperatures 80 °C and 60 °C for Cel776Ec and 60 °C for Cel776Sc. C Thermal stability at 60 °C, 70 °C and 80 °C for long incubation times (1 h to 24 h) for Cel776Ec and D thermal stability at 70 °C, 80 °C and 90 °C for short incubation times (1 min to 60 min) for Cel776Ec (green) and Cel776Sc (red). E Effect of additives at 5 mM concentration on the enzymatic activity. An asterisk represents a p value lower than 0.05 in a one way ANOVA test for the control and each condition. Two represent a p value lower than 0.005; three lower than 0.0005 and four lower than 0.0001. F Activity towards alternative substrates. NM: not measured