Fig. 1

Molecular identification of different T3 BnLPAT2 and BnLPAT5 lines. a CRISPR–Cas9 induced target mutations in the T3 progenies of BnLPAT2&5 knockout plants. Exons are shown as rectangles, whereas introns are shown as black lines. Target sites are located in conserved CDS regions. PAMs are in green, and gRNAs are in yellow. SNPs are highlighted in light red. “ + ” and “ − ” indicate nucleotide insertions and deletions in the target sequences, respectively; “s” indicates substitutions; “c” indicates combinatorial mutations; and “cv” indicates complicated variant. b Relative gene expression levels of BnLPAT2&5 in the corresponding overexpression, RNAi, and Cas9 lines