Fig. 2

Analysis of the SOCs and morphologies of the mature seeds of different BnLPAT2 and BnLPAT5 transgenic lines. a Seed morphology of the dry mature seeds of different BnLPAT2 and BnLPAT5 transgenic lines. The seeds are laid out from the largest to the smallest in terms of area. b TSWs of different BnLPAT2 and BnLPAT5 transgenic lines. c, d SOCs of the T2 and T3 progenies of different BnLPAT2 and BnLPAT5 transgenic lines. The small black dots in c and d represent the SOCs of different biological samples. LP2-G123 and LP2&LP5-G134 are multi-gRNA-mediated knockouts of BnLPAT2 and BnLPAT5 from G1 to G4 as described in a previous work [32]. LP2-OE&LP5-OE represents the hybrid lines of LP2-OE and LP5-OE, and lp2&lp5 represents the hybrid lines of Bnlpat2 and Bnlpat5 knockout or knockdown lines. Mann–Whitney test was performed on b, c, and d, and significant differences between transgenic lines and the WT at P < 0.05 (*) and P < 0.001 (**) are shown