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Table 1 Fermentation data for various recombinant strains

From: Acetoin production from lignocellulosic biomass hydrolysates with a modular metabolic engineering system in Bacillus subtilis

Strain

Acetoin

(g/L)

2,3-Butanediol

(g/L)

Lactic acid

(g/L)

Succinic acid

(g/L)

Residual glucose

(g/L)

Equilibrium yield

(mol/mol)

BSMAY-1

34.1 ± 0.3

6.2 ± 0.54

2.3 ± 0.1

1.4 ± 0.1

1.0 ± 0.2

0.70 ± 0.01

BSMAY-2

36.5 ± 0.8

1.7 ± 0.32

2.9 ± 0.2

1.4 ± 0.1

1.0 ± 0.3

0.75 ± 0.01

BSMAY-3

38.2 ± 0.5

2.0 ± 1.0

0.1 ± 0.1

1.6 ± 0.1

1.0 ± 0.2

0.78 ± 0.01

BSMAY-4

40.1 ± 0.2

1.9 ± 0.7

0.2 ± 0.1

0.5 ± 0.1

1.0 ± 0.3

0.82 ± 0.01

  1. All assays are performed in triplicate and the standard deviation of biological replicates is expressed as a numerical error (note: the theoretical maximum of acetoin production per 100 g/L of glucose is 48.89 g/L, equilibrium yield = titer of acetoin/48.89 g/L)