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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Inhibition of monogalactosyldiacylglycerol synthesis by down-regulation of MGD1 leads to membrane lipid remodeling and enhanced triacylglycerol biosynthesis in Chlamydomonas reinhardtii

Fig. 3

Relative gene expression analysis using quantitative real-time PCR. a DAG biosynthesis. b TAG biosynthesis. c ROS scavenging. d ER stress marker. Relative expression levels were calculated by normalizing the values to those of Chlamydmonas β-subunit-like polypeptide (CBLP). Significant differences between WT and Cr-mgd1 are represented with asterisks based on Holm–Sidak method (**P < 0.01), and the error bars represent the standard deviation (SD). WT: wide-type; DGAT1: diacylglycerol O-acyltransferase1; PDAT: phospholipid:diacylglycerol acyltransferase; LCS2: long-chain acyl-CoA synthetase2; MLDP: major lipid droplet protein; PGD1: plastid galactiglycerolipid degradation1; SOD: superoxide dismutase; CAT1: catalase1; APX1: ascorbate peroxidase1; GPX5: glutathione peroxidase5; GPAT1: glycerol 3-phosphate; CrLPAAT2: 2‐lysophosphatidic acid acyltransferase2 from Chlamydomonas reinhardtii; PAP2: phosphatidic acid phosphatase2; BiP1: binding protein1; CAL2: calreticulin2; CrIRE: Chlamydomonas reinhardtii inositol-requiring enzyme1

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