Fig. 2From: Both levoglucosan kinase activity and transport capacity limit the utilization of levoglucosan in Saccharomyces cerevisiaeGrowth of recombinant strains of S. cerevisiae on SC-URA medium supplemented with 5 g L−1 levoglucosan as the sole carbon source. Strains were pre-cultured overnight, transferred to fresh media and cultured for another 12 h. Cells were harvested and resuspended by sterile ddH2O until the OD600 of the suspension reached 1.0, and then a series of tenfold dilutions were spotted onto SC-URA media plates supplemented with 5 g L−1 levoglucosanBack to article page