Table 2 Primers used in this study
Name | Sequence (5ʹ to 3ʹ) | Purpose of primer | Expected size | Cycles/Tm | Reference |
|---|---|---|---|---|---|
Km_FKpnI | TAGAGAGGTACCTTAGAAAAACTCATCGAG CA | PCR for kmr | 939 | 30/60.0 °C | This study |
Km_RKpnI | TAGAGAGGTACCGTGTCTCAAAATCTCTGATG | PCR for kmr | This study | ||
Km_SR | TAGAGATCAGTCGTCACTCATGGTGA | PCR for kmr | Â | Â | This study |
Sll1951_F | TAGAGAGTGGAAGATGCAAAT ATACT | PCR for sll1951 | 1980 | 35/56.3 °C | This study |
Sll1951_R | TAGAGAGGCGCTATCACT GGTAAAAG | PCR for sll1951 | This study | ||
Sll1951_UF | TAGAGAGTGGAAATTGCG GCTTCC CT | PCR for sll1951 | Â | Â | This study |
RTglgX_F360 | GAGCTTCATCGAGGACGGAA | RT-PCR for glgX | 360 | 35/56.0 °C (BG11) | This study |
RTglgX_R360 | GCCCGAATTGGGGTTGCGGG | RT-PCR for glgX | 30/56.0 °C (BG11-N) | ||
RTphaA_F420 | TCAGCCGGATAGAATTGGACG AAGT | RT-PCR for phaA | 420 | 35/53.5 °C (BG11) | [8] |
RTphaA_R420 | CAAACAAGTCAAAATCTGCCA GGGTT | RT-PCR for phaA | 30/53.5 °C (BG11-N) | ||
RTlipA_F379 | TTGGCGGAGCAAGTGAAGCAAT | RT-PCR for lipA | 379 | 34/55.0 °C (BG11) | [8] |
RTLipA_R379 | CATGGACCAGCACAGGCAAAAT | RT-PCR for lipA | 28/55.0 °C (BG11-N) | ||
RTaccA_F428 | ATGCACGGCGATCGAGGAGGT | RT-PCR for accA | 428 | 35/58.0 °C (BG11) | [8] |
RTaccA_R428 | TGGAGTAGCCACGGTGTACAC | RT-PCR for accA | 32/58.0 °C (BG11-N) | ||
RT16sRNA_F521 | AGTTCTGACGGTACCTGATGA | RT-PCR for 16 s | 521 | 24/56.0 °C (BG11) | [8] |
RT16sRNA_R521 | GTCAAGCCTTGGTAAGGTTAT | RT-PCR for 16 s | 22/56.0 °C (BG11-N) | ||
RTaas_F307 | GTGGTTTATCGCCGATCAAG | RT-PCR for aas | 307 | 38/54.5 °C (BG11) | [8] |
RTaas_R307 | TTCCTGGCGGGGAACGGGAG | RT-PCR for aas | 33/54.5 °C (BG11-N) | ||
RTPlsX_F | AAGGGGTGGTGGAAATGGAA | RT-PCR for PlsX | 488 | 35/52.7 °C (BG11) | [6] |
RTPlsX_R | AAGTAGGTCCCTTCCTTCGG | RT-PCR for PlsX | 32/52.7 °C (BG11-N) |