Table 6 Strains and plasmids used in this study
Strain or plasmid | Description | Source |
|---|---|---|
Strains | Â | Â |
E. coli DH5α | Gene cloning of host bacteria | Transgen |
MTL01 | SO26 with deletion of mtlR | [21] |
MTL13 | l-Ornithine producing strain derived from C. glutamicum S9114 | |
MTL14 | MTL13 with Psod promoter inserted in the upstream region of qsuR | This study |
MTL15 | MTL13 with Psod promoter inserted in the upstream region of prpC | This study |
MTL16 | MTL13 with Psod promoter inserted in the upstream region of CGS9114_RS08985 | This study |
MTL17 | MTL13 with terminator inserted in the upstream region of acnR | This study |
MTL18 | MTL13 with terminator inserted in the upstream region of CGS9114_RS09730 | This study |
MTL19 | MTL13 with terminator inserted in the upstream region of pdxR | This study |
MTL20 | MTL14 with Psod promoter inserted in the upstream region of prpC | This study |
MTL21 | MTL14 with Psod promoter inserted in the upstream region of CGS9114_RS08985 | This study |
MTL22 | MTL14 with terminator inserted in the upstream region of acnR | This study |
MTL23 | MTL14 with terminator inserted in the upstream region of CGS9114_RS09730 | This study |
MTL24 | MTL14 with terminator inserted in the upstream region of pdxR | This study |
MTL25 | MTL24 with Psod promoter inserted in the upstream region of CGS9114_RS08985 | This study |
Plasmid | Â | Â |
pK18mobsacB | Mobilizable vector, allows for selection of double crossover in C. glutamicum, KmR, sacB | Laboratory stock |
pK18-T- CGS9114_RS09730 | A derivative of pK18mobsacB, harboring T- CGS9114_RS09730 fragment | This study |
pK18-T-pdxR | A derivative of pK18mobsacB, harboring T- pdxR fragment | This study |
pK18-T-acnR | A derivative of pK18mobsacB, harboring T-acnR fragment | This study |
pK18-Psod-qsuR | A derivative of pK18mobsacB, harboring Psod-qsuR fragment | This study |
pK18-Psod-prpC | A derivative of pK18mobsacB, harboring Psod-prpC fragment | This study |
pK18-Psod- CGS9114_RS08985 | A derivative of pK18mobsacB, harboring Psod-CGS9114_RS08985 fragment | This study |