Fig. 1From: Engineering sigma factors and chaperones for enhanced heterologous lipoxygenase production in Escherichia coliConstruction of the screening system for soluble LOX expression. A Schematic diagram of the self-assembly protein–protein interaction of different domains in GFP (left). Soluble expression system with spontaneous assembly LOX fluorescence (right). B Fluorescence density analysis of the domain of split-GFP. C Fluorescence analysis of the soluble fraction of the self-assembly protein. D Enzyme activity and insoluble fraction fluorescence analysis using different screening systems. E Analysis of the expression level of LOX in different soluble expression systemsBack to article page