Fig. 4

Supply of acetyl-CoA driven by degradation of TAG in LD metabolism-engineered strains. A Acetyl-CoA and B TAG levels of the LD metabolism-engineered strains. The acetyl-CoA level of the LD metabolism-engineered strains slightly increased with a concomitant decrease in TAG levels compared to those of the control SQ4td strain. C Quantification of the size and number of LDs in the LD metabolism-engineered strains. The graph depicts scatter plots of morphological changes in the LDs of the LD metabolism-engineered strains (n = 6). For the scatter plots, medians with interquartile ranges are displayed. The numbers in parentheses indicate the number of LDs in each engineered strain. D Differential interference contrast and confocal fluorescence microscopy images of the LDs in the engineered yeast strains. All strains were cultured in shake flasks with YSC medium containing 2% (w/v) glucose for 144 h at 30 °C. All the data represent the mean of biological triplicates, and error bars indicate the standard deviation. An asterisk indicates that the value is significantly different (*P < 0.05 and **P < 0.01) from that of the respective control SQ4td strain. Scale bar: 2.5 μm