Fig. 2
Schematic representation of the Synechocystis PCC 6803 strains engineered in this study. Synechocystis cells are shown as green oval shapes representing their chromosome (orange line) arbitrarily showed as attached to the cell membrane to distinguish it from the pC plasmids. Most strains harbor a derivative of the SmR/SpR replicative plasmid vector pC (grey line) expressing a terpene-synthase encoding gene from the strong λ phage pR promoter (red triangle). These plasmids are: pCLS (limonene synthase, yellow arrow), pCPS, (pinene synthase, brown arrow), pCBS (bisabolene synthase, blue arrow), pCSS (santalene synthase, pink arrow) and pCFS (farnesene synthase, green arrow). Note that two other strains express the same pR-FS gene cassette from their chromosome (precisely from the slr0168 site, where it was introduced along with the KmR marker gene). They served for the comparison of farnesene production driven by the pR-FS gene expressed from either of both the pCFS plasmid and the chromosome