Fig. 3
From: Arabinan saccharification by biogas reactor metagenome-derived arabinosyl hydrolases
Cloning and expression of seven putative arabinosyl hydrolases from the enzyme families GH51 and GH43. The coding regions were identified in the sequence of the assembled DNA fragment shown in Fig. 1. A PCR amplification of the putative arabinosyl hydrolase-encoding genes was done using fosmid clone MOD18_Abn4 as a template. Lane 1: GeneRuler 1 kb, Lane 2: MC57GH51 (1,500 bps); Lane 3: MC72GH43-1 (1,407 bps); Lane 4: MC72GH43-2 (2,580 bps); Lane 5: MC60GH43 (960 bps); Lane 6: MC60GH51 (1,533 bps); Lane 7: MC68GH43-1 (1,575 bps); Lane 8: MC68GH43-2 (1,002 bps). B SDS-PAGE analysis of the purified recombinant arabinosyl hydrolases. The proteins were purified by immobilized metal affinity chromatography with a Ni-TED packed column and subsequent anion-exchange chromatography with a QFF column. Lane 1: prestained protein standards, Lane 2: MC57GH51 (56.65 kDa); Lane 3: MC60GH51 (58.40 kDa); Lane 4: MC68GH43-1 (58.80 kDa); Lane 5: MC60GH43 (36.71 kDa); Lane 6: MC68GH43-2 (39.23 kDa); Lane 7: MC72GH43-2 (95.88 kDa); Lane 8: MC72GH43-1 (52.18 kDa)