Arabinan saccharification by biogas reactor metagenome-derived arabinosyl hydrolases

Table 2 Summary of “non-E. coli” contigs from shotgun sequencing of positive fosmid clones

Retrieved non-E. coli contigs^a	pCC1FOS fosmid pool (40 clones)	pCT3FK fosmid pool (15 clones)	Both pools
Number of contigs	39	42	81
Number of retrieved contigs^b	32	22	54
No. of contigs with CAZymes^c	13	18	31
Total length of contigs (bps)	295,166	344,179	639,345
Average length of contigs (bps)^d	7568	8194	7893

Positive clones were screened by using chromogenic polysaccharide hydrogel (CPH) substrates and insoluble chromogenic biomass (ICB) substrates
^aContigs from E. coli genomic DNA contamination or from vector backbone were confirmed by the size of contig (far more than 40 kb) and sequence identity searched against NCBI database
^bRetrieved contigs refer to the contigs which could be aligned to at least one Sanger end sequence
^cCAZymes were predicted by dbCAN database
^dAverage length of contigs was calculated by using the sum of the contig lengths divided by the amount of contigs after filtering out the E. coli originated contigs

ISSN: 2731-3654