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Table 5 Specific activities and kinetic parameters of the characterized arabinosyl hydrolases on DA and SBA

From: Arabinan saccharification by biogas reactor metagenome-derived arabinosyl hydrolases

 

Specific activitya

Released soluble sugarsb

Enzymes

SBA

DA

MC57GH51

2.02 ± 0.11; Km = 117.02, vmax = 52.67

ND

Arabinose

MC60GH51

0.06 ± 0.00; Km and vmax: ND

0.15 ± 0.01; Km and vmax: ND

Arabinose

MC60GH43

NA

1.65 ± 0.17; Km = 83.05, vmax = 0.82

Arabinose

MC72GH43-1

NA

NA

NA

MC72GH43-2

3.81 ± 0.25; Km = 14.98, vmax = 22.77

5.21 ± 0.21; Km = 16.61, vmax = 18.27

AOS, Arabinose

MC68GH43-1

7.51 ± 0.31; Km = 9.25, vmax = 2.96

2.39 ± 0.26; Km = 11.04, vmax = 23.07

AOS, Arabinose

MC68GH43-2

ND

1.74 ± 0.02; Km = 37.44, vmax = 14.02

Arabinose

  1. Km in g L−1, vmax in µmol min−1 mg−1, Km and vmax were calculated by Microsoft Excel Solver based on enzyme activities at their optimal conditions with different substrate concentrations. ND means not determined due to insufficient activity or lack of detectable activity. NA means no activity
  2. aSpecific activity in µmol min−1 mg−1. The data show the mean values and standard deviations from three experiments
  3. bSoluble sugars released, analyzed by HPAEC-PAD and TLC
  4. SBA sugar beet arabinan, DA debranched arabinan, AOS arabino-oligosaccharides
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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