Fig. 3

Characterization of the Pt2015 knockout strain (2015KO). A Characteristics of the 2015KO. Sequences of the 2015KO strain and wild type (WT) were aligned. The mutation in the Pt2015 locus compared to the WT sequence was indicated, in which 16 bp are deleted. B Quantitative reverse transcription polymerase chain reaction (qRT–PCR) analysis of Pt2015 mRNA levels in the WT, 2015KO, and the Pt2015 overexpression (oeT) strains. Ribosomal protein S (RPS) was used as reference gene. Error bars represent the standard deviation (n = 3). C Immunoblotting analysis of the Pt2015 protein in the WT, 2015KO, and oeT strains. Photosystem II protein D1 (PsbA) protein level was detected as the loading control. Fifteen micrograms protein extracts of the WT, 2015KO, and oeT strains have been loaded