Table 1 Strains and plasmids used in this study
Strains | Description | Reference |
|---|---|---|
2pLimA | E. coli DH1 with plasmids JPUB_017011 + JPUB_017013 | [42] |
DM01 (JBEI-15051) | E. coli DH1 with S81F mutation in ispA | [3] |
DM02 (JBx_136491) | DM01 with plasmids JBEI-15240 + JBEI-15060 | [3] |
DM03 (JBEI-15050) | E. coli MG1655 with mutation (S81F) in ispA | This study |
DM04 (JBx_136471) | DM03 with plasmids JBEI-15240 + JBEI-15060 | This study |
DM05 (JBx_136467) | DM01 with deletions ΔpoxB Δpta ΔackA | This study |
DM06 (JBx_136472) | DM03 with deletions ΔpoxB Δpta ΔackA | This study |
DM07 (JBx_136468) | DM05 with plasmids JBEI-15240 + JBEI-15060 | This study |
DM08 (JBx_136470) | DM06 with plasmids JBEI-15240 + JBEI-15060 | This study |
DM09 (JBx_136473) | DM06 with deletion ΔldhA | This study |
DM10 (JBx_136474) | DM09 with plasmids JBEI-15240 + JBEI-15060 | This study |
DM11 (JBx_137160) | DM09 with plasmid JBx_136481 + JBEI-15060 | This study |
DM12 (JBx_137158) | DM09 with plasmids JBEI-15240 + JBx_136482 | This study |
DM13 (JBx_137159) | DM09 with plasmids JBx_136481 + JBx_136482 | This study |
Plasmids | Description | Reference |
|---|---|---|
JPUB_017011 | pBbA5a-MTSA-T1-MBI; 7 genes of the MVA pathway (from thiolase to IPP isomerase) for IPP and DMAPP production | [42] |
JPUB_017013 | pBbE1k-trGPPS-LS; truncated GPP synthase and limonene synthase under Trc promoter | [42] |
JBEI-15240 | pBbA5c-MTSA-T1-MBI-T1002-Ptrc-CSStr; JPUB_17011 with bacterial cineole synthase added under Trc promoter | [3] |
JBEI-15060 | pTrc99a-GPPS-CSStr-ispA*; GPP synthase and bacterial cineole synthase under Trc promoter | [3] |
JBx_136481 | pBbA5c-MTDa-T1-MBI-T1002-Ptrc-CSStr | This study |
JBx_136482 | pTrc99a-GPPS-CS-ispA(S81F)-HMGR_Da | This study |