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Table 1 Specific activity of exo-1,3-β-glucanase G9376 toward different substrates

From: Characterization of two 1,3-β-glucan-modifying enzymes from Penicillium sumatraense reveals new insights into 1,3-β-glucan metabolism of fungal saprotrophs

Substrate

Specific activity (U/mg)

pNPGlc

–

pNPGal

–

pNPLAM2

–

PGA

–

CMC

0.053 ± 0.005

Arabinoxylan

–

LAM5ol

18.9 ± 0.05

Laminarin

72.1 ± 0.23

  1. Enzyme activity, expressed as Units (µmol/min) per mg of enzyme, was evaluated at 25 °C and pH 5. Units are expressed as µmol reducing ends (for PGA, CMC, Arabinoxylan, LAM5ol and laminarin substrates) and µmol p-nitrophenol (for pNPGLc, pNPGal and pNPLAM2 substrates) released per minute. Values are mean ± SD, n = 3; — no activity detected
  2. pNPGlc p-nitrophenyl-β-D-glucopyranoside, pNPGal p-nitrophenyl-β-D-galactopyranoside; pNPLAM2 p-nitrophenyl-β-D-laminaribioside, CMC carboxymethyl-cellulose, PGA polygalacturonic acid, LAM5ol 1,3-β-D-laminaripentaitol borohydride
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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