Second generation Pichia pastoris strain and bioprocess designs

Ergün, Burcu Gündüz; Laçın, Kübra; Çaloğlu, Buse; Binay, Barış

doi:10.1186/s13068-022-02234-7

Biotechnology for Biofuels and Bioproducts

Table 2 Pichia pastoris cell growth and bioproduct generation with a variety of wastes and by-products

From: Second generation Pichia pastoris strain and bioprocess designs

Source	Strain	Promoter	Product	Bioreactor	Bioprocess operation mode	Yield	Productivity	References
Agri-food industry by-products and wastes
Wood sugar xylose
Corncob hydrolysate	GS115 and evolved strain (GS30)	–	β-Galactosidase β-Mannanase	Shake flask	Fed-batch process	72.6 U/mL 45.1 U/mL	–	[52]
Xylose	PichiaPink	P_MOX	Endoglucanase 3 EndoglucanaseII	Shake flask	-	No expression	–	[54]
Xylose	GS115	P_GAP	β-Mannanase	Shake flask	Batch process	> 60 U/mL	–	[104]
Cereal by-products
Pakchong 1	–	–	Xylanases	Shake flask	SoSF	31.44 ± 0.88 U/g	0.2620 ± 0.02 U/g.h⁻¹	[80]
Sugarcane leaves	–	–	Xylanases	Shake flask	SoSF	29.72 ± 0.35 U/g	0.2477 ± 0.01 U/g.h⁻¹	[80]
Merkeron	–	–	Xylanases	Shake flask	SoSF	34.10 ± 0.88 U/g	0.2842 ± 0.01 U/g.h⁻¹	[80]
Alafal	–	–	Xylanases	Shake flask	SoSF	26.84 ± 0.92 U/g	0.2237 ± 0.09 U/g.h⁻¹	[80]
Alafal	–	–	Xylanases	Shake flask	SmF	56.08 ± 0.34 U/g	0.4674 ± 0.03 U/g.h⁻¹	[80]
Merkeron	–	–	Xylanases	Shake flask	SmF	49.37 ± 0.72 U/g	0.4114 ± 0.05 U/g.h⁻¹	[80]
Pakchong 1	–	–	Xylanases	Shake flask	SmF	49.48 ± 0.30 U/g	0.4124 ± 0.05 U/g.h⁻¹	[80]
Sugarcane leave	–	–	Xylanases	Shake flask	SmF	61.21 ± 0.52 U/g	0.5101 ± 0.03 U/g.h⁻¹	[80]
Merkeron	–	–	Cellulase	Shake flask	SoSF	11.85 ± 0.35 U/g	0.0988 ± 0.01 U/g.h⁻¹	[80]
Sugarcane leaves	–	–	Cellulase	Shake flask	SoSF	10.37 ± 0.49 U/g	0.0864 ± 0.05 U/g.h⁻¹	[80]
Alafal	–	–	Cellulase	Shake flask	SoSF	8.89 ± 0.61 U/g	0.0618 ± 0.03 U/g.h⁻¹	[80]
Pakchong 1	–	–	Cellulase	Shake flask	SoSF	9.44 ± 0.37 U/g	0.0787 ± 0.02 U/g.h⁻¹	[80]
Merkeron	–	–	Cellulase	Shake flask	SmF	39.21 ± 0.45 U/g	0.3268 ± 0.03 U/g.h⁻¹	[80]
Sugarcane leaves	–	–	Cellulase	Shake flask	SmF	39.72 ± 0.30 U/g	0.3310 ± 0.02 U/g.h⁻¹	[80]
Alafal	–	–	Cellulase	Shake flask	SmF	29.51 ± 0.65 U/g	0.2459 ± 0.01 U/g.h⁻¹	[80]
Pakchong 1	–	–	Cellulase	Shake flask	SmF	31.37 ± 1.16 U/g	0.2614 ± 0.02 U/g.h⁻¹	[80]
Crude glycerol, Soybean hydrolysate and Rice bran	KM71	P_AOX1	Xylanase	Shake flask	Methanol fed-batch process	1383.9 U/mL	–	[82]
Wheat bran hydrolysate	–	P_AOX1	Xylanase B	Shake flask	Methanol fed-batch	1059.8 U/mL	–	[103]
Maize meal bean pulp	GS115	P_AOX1	Xylanase	Shake flask	Methanol fed-batch process	3273.0 U/mL	–	[81]
Wheat bran	–	P_AOX1	Xylanase	Bioreactor	Methanol fed-batch process	3,683 mU/mL	–	[79]
Wheat bran	X33	P_AOX1	Xylanase	Bioreactor	Methanol fed-batch process	560.7 U/mL	–	[78]
Corncob	X33	P_AOX1	Xylanase	Shake flask	Methanol fed-batch process	Decreased by the rate of 70.31%	–	[78]
Cottonseed hull	X33	P_AOX1	Xylanase	Shake flask	Methanol fed-batch process	Positive effect	–	[78]
Corn bran	X33	P_AOX1	Xylanase	Shake flask	Methanol fed-batch process	Positive effect	–	[78]
Corn processing wastewater	GS115	P_AOX1	Lipase	Shake flask	Methanol fed-batch process	163 U/L	–	[77]
Sugar industry by-products
Miscellaneous waste carbohydrates of high fructose syrup	–	P_GAP P_AOX1	Endo-β-1,3-glucanase	Bioreactor	Fed-batch process	171.8 U/ml 212.3 U/mL	–	[105]
Sugarcane bagasse	X33	P_GAP	Xylonic acid	Shake flask	Batch process	0.43 ± 0.06 g/g	0.16 ± 0.02 g/L.h	[106]
Sugar beet molasses	–	P_GAP	Recombinant human growth hormone	Bioreactor	Fed-batch process	625 mg/dm³	–	[107]
Molasses	GS115	P_FLD1	α-Amylase	Bioreactor	Methanol Fed-batch process	120 mg/L	–	[108]
Biodiesel industry by-products and wastes
Crude glycerol	BG-10, BG-11, BG-16	P_D915	Laccase	Shake flask	Batch process	2343 ± 60 U/L	–	[109]
Crude glycerol	SMD1168H	P_GAP	Lectin	Bioreactor	Glycerol fed-batch process	265 ± 13 mg/L	2.04 mg/L.h	[110]
Crude glycerol	GS115	P_AOX1	Lipase	Shake flask	Methanol fed-batch process	1437 U/mL (15,977 U/mg)	–	[111]
Sago bioethanol liquid waste	GS115	P_AOX1	Laccase	Shake flask	Methanol fed-batch process	0.00159 U/ mL	–	[112]
Crude glycerol	X33	P_AOX1	β-Mannanase	Bioreactor	Methanol fed-batch process	10,900 U/mL	–	[113]
Crude glycerol	X33	P_PGK1	Lipase	Bioreactor	Glycerol fed-batch process	50,041 U/L	–	[114]
Waste glycerol	X33	–	–	Bioreactor	Batch process	Positive effect on growth	–	[115]
Glycerin byproduct	X33	P_PGK	α-Amylase	Bioreactor	Batch process	̴ 3.5 U/mL	–	[116]
Waste glycerol	GS115	–	–	Shake flask	Batch process	Positive effect on growth	–	[117]
Co-culture studies for valorisation of wastes and by-products
Kitchen waste	GS115 co-culture with Bacillus amyloliquefaciens	P_AOX1	Amylase	Shake flask	Methanol fed-batch process	385.4 U/mL	–	[118]
Kitchen waste	GS115 co-culture with Bacillus amyloliquefaciens	P_AOX1	Glucosidase	Shake flask	Methanol fed-batch process	247.3 U/mL	–	[118]
Kitchen waste	GS115 co-culture with Bacillus amyloliquefaciens	P_AOX1	Lipase	Shake flask	Methanol fed-batch process	90 U/mL	–	[118]
Xylose	GS115 co-culture with S. cerevisiae	P_AOX1	β-Xylosidase	Shake flask	Methanol fed-batch process	3.02 ± 0.030 U/mL	–	[37]
Xylose	GS115 co-culture with S. cerevisiae	P_AOX1	Endo-1,4-β-d-xylanase	Shake flask	Methanol fed-batch process	1.52 ± 0.035 U/mL	–	[37]
Wheat straw	GS115 co-culture with S. cerevisiae	P_AOX1	Ethanol	–	Methanol fed-batch process	32.6 g/L	–	[37]
Metabolic engineering studies for low-cost substrate utilization
Sugarcane trash hydrolysates	KM71	P_GAP	Isobutanol	Shake-flask	Batch process	48.2 ± 1.7 mg/L	–	[119]
CO₂	CBS7435	P_AOX1, P_DAS1 P_DAS2	Growth	Bioreactor	Fed-batch process	µ was increased from from 0.008/h to 0.018/h	–	[120]
Crude hemicellulose hydrolysate	GS115	P_AOX1	Formate dehydrogenase Xylose reductase Glucose dehydrogenase	Bioreactor	Methanol fed-batch process	0.34 ± 0.02 U/mg 62.1 ± 7.1 U/mg 17.4 ± 1.0	–	[121]
Acetate	GS115	P_GAP	6-Methylsalicylic acid	Shake flask	Batch process	Enhanced	–	[122]
Cellobiose -carboxymethyl cellulose	BG10	P_AOX1	Endoglucanase, β-glucosidase Exoglucanase	Shake flask	Batch process	Positive effect	–	[123]
Xylan, carboxymethyl cellulose	X33	P_ADH2	Chimeric endoglucanase Xylanase	Shake flask	Batch process	1.4 U/mg 1.8 U/mg	–	[124]

SoSF solid-state fermentation, SmF submerged fermentation

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