Fig. 2
From: Towards the rate limit of heterologous biotechnological reactions in recombinant cyanobacteria
Whole-cell Biotransformation of 1a mediated by Syn::PcpcBYqjM. Time course of product formation in BG-11 medium with and without addition of D-Glu (2.5 mM, added at the start of the reaction) at (A) OD750 = 2.5 corresponding to 0.6 gDCW L−1 and at (B) OD750 = 10 corresponding to 2.4 gDCW L−1. C Specific Activities from the reduction of 1a at different optical densities and addition of D-Glu. HL = high light cultivation and low light reaction; LL = low light cultivation and reaction; HH = high light cultivation and reaction; LH = low light cultivation and high light reaction. Reaction conditions: V = 1.2 mL, T = 30 °C, 160 rpm, Initial concentration of 1a = 10 mM, High light intensity = 200 µmol photons m−2 s−1, Low light intensity = 40–60 µmol photons m−2 s−1, N = 3. P values were calculated using Welch´s t test (*P ≤ 0.05; **P ≤ 0.005; n.s. = not significant) and correspond to the specific activity comparisons between reactions performed with and without D-Glu under light and darkness