Fig. 3

Stimulating effect of D-Glu in the reduction of various substrates mediated by Syn::P_cpcBYqjM. (A) Time course of product formation and (B) Specific activities using various substrates mediated by Syn::P_cpcBYqjM. Reaction conditions: V = 1.2 mL, T = 30 °C, 160 rpm, Initial concentration of 1a = 10 mM, Light intensity = 200 µmol photons m⁻² s⁻¹, D-Glu = 2.5 mM (added at the start of the reaction), N = 3. Control reactions were performed in the absence of D-Glu. P values were calculated using Welch´s t test (*P ≤ 0.05; ***P ≤ 0.0005) and correspond to the specific activity comparison between control experiments and with D-Glu addition