Fig. 1

Construction and validation of the xyr1 overexpression strains. A Schematic diagrams of the expression cassettes used for differential overexpression of the xyr1 gene. The overexpression of xyr1 was controlled by three different promoters (Pegl2, Pcbh1, and Pcdna1), respectively. B Transcript levels of xyr1 which was determined by RT-qPCR in QEB4 and three independent transformants of each xyr1 overexpression strain (QE2X, QCBX, and QCDX). Strains were cultivated at 30 ℃ and 200 rpm in flasks using the CPM medium with 2% (w/v) Avicel as the sole carbon source for 72 h. The transcript level was normalized to that of actin (t-test, **p < 0.01). Results are means of three biological replicates and error bars indicate ± SD