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Table 3 Production and thermal stability of C-leB single mutants

From: Tailor-made alkaliphilic and thermostable fungal laccases for industrial wood processing

Variant

Mutation

Activity (U/L)

T50 (10 min) °C

C-LeB

–

1812

74 ± 0.2

207

S207N

324

–

291

S291T

1765

74 ± 0.2

413

T413V

1850

74 ± 0.1

457

D457E

1798

75 ± 0.2

461

A461T

2230

69 ± 0.1

468

T468I

1839

74 ± 0.03

  1. Activities (with ABTS, pH 3) detected in the liquid extracts of S. cerevisiae cells expressing C-LeB and its single-mutated variants cultured in flasks, and T50 (10 min) values of the laccase variants
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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