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Table 5 Half-lives of RY2 parent laccase and its last evolved alkaliphilic and thermophilic variants

From: Tailor-made alkaliphilic and thermostable fungal laccases for industrial wood processing

T (°C)

t1/2 (h)

RY2

C-LeB

Mol2

Mol3

60 °C

2.0

1.3

5.5

10.2

70 °C

0.4

0.6

0.8

1.5

  1. Residual activities of the pure enzymes were determined with ABTS, pH 3