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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Regulation of seed oil accumulation by lncRNAs in Brassica napus

Fig. 4

The co-expression analysis of lncRNAs and mRNAs and the selection of candidate lncRNAs. A Co-expression network analysis in cluster 2. Green circles represent lncRNA, blue circles represent mRNA, the yellow circle is MSTRG.22563. B Co-expression network analysis in cluster 4. Green circles represent lncRNA, blue circles represent mRNA, the yellow circle is MSTRG.86004. C and D, The expression levels of MSTRG.22563 and MSTRG.86004 from RNA-seq data and qRT-PCR data. Relative expression level in qRT-PCR was calculated by comparison to actin’s expression level. Values are means ± SD (n = 3). E and F, The distribution and expression patterns of MSTRG.22563 and MSTRG.86004 in 20 and 40 DAF seeds of four B. napus accessions in ATAC-seq analysis. The peaks representation of the expression of transcripts, color figures representation of the expression of lncRNAs in the region, black figures representation of the expression of mRNAs in the region. The structure colored with bottle-green represents genes, the structure colored with yellow represents lncRNAs. ATAC-seq, Assay for Transposase-Accessible Chromatin with high-throughput sequencing

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