Fig. 2

Effect of agitation speed on the cell growth and metabolite production by K. pneumoniae GEM167ΔadhE/pBR-1,2PDO. A glycerol consumption; B growth (OD_{600 nm}); C 1,2-PDO concentration; D lactic acid concentration; E acetic acid concentration; F 1,3-PDO concentration; G 2,3-BDO concentration; H ethanol concentration; I succinate concentration. Cultivation was in a 5-L jar fermenter (37 °C, 0.5 vvm, pH 6 maintained using 28% v/v NH₄OH) for 48 h with glycerol as carbon source. Black circles, 100 rpm; red triangles, 200 rpm; blue diamonds, 300 rpm; green squares, 400 rpm. Here and below: means ± SDs of triplicate measurements are shown. SD, standard deviation