Table 5 Metabolite analysis of the K. pneumoniae GEM167ΔadhE/pBR-1,2PDO strain after fed-batch cultivation at different pH values for 48 h
pH | Maximum metabolite concentrations | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Glycerol Consumption (g/L) | Cell growth (OD 600Â nm) | 1,2-PDO (mg/L) | 2,3-BDO (g/L) | Lactic acid (g/L) | Acetic acid (g/L) | Succinate (g/L) | Ethanol (g/L) | 1,3-PDO (g/L) | |
pH 6 | 158.0 ± 1.1 | 18.4 ± 0.7 | 731.0 ± 24.7 | 51.9 ± 2.3 | 16.5 ± 1.4 | 14.1 ± 0.6 | 1.2 ± 0.2 | 1.7 ± 0.1 | 0.2 ± 0.1 |
pH 7 | 126.3 ± 1.3 | 22.8 ± 0.5 | 1016.5 ± 37.3 | 9.2 ± 1.6 | 72.2 ± 1.3 | 24.3 ± 0.9 | 4.3 ± 0.1 | 2.0 ± 0.2 | 0.2 ± 0.1 |
pH 8 | 92.2 ± 1.2 | 15.8 ± 0.6 | 141.2 ± 29.1 | 0.4 ± 0.2 | 45.5 ± 1.8 | 27.1 ± 0.7 | 0.8 ± 0.1 | 1.6 ± 0.1 | 0.1 ± 0.1 |