Fig. 10
Production of N-Acetyl glucosamine (GlcNAc) by the fermentation supernatant of the chitinolytic enzymes expression strain A. niger FCN30. A Reaction mixtures containing 1% colloidal chitin as substrate in 50 mM phosphate–citrate buffer (pH 5.0) were incubated with the fermentation broth of FCN30 (left) and ATCC 20611 (right) at 50 ℃ for 24 h. B Thin-layer chromatography (TLC) analysis of hydrolysis products of colloidal chitin by FCN30 (left) and ATCC 20611 (right) at different time intervals. Standard N-acetyl-chitooligosaccharides ranging from GlcNAc to (GlcNAc)6 were used as the marker. B Quantification of GlcNAc produced during the degradation of colloidal chitin by HPLC. Error bars represent the standard deviation (SD) of three replicates. Values are the average of the triplicates and error bars are the standard deviation (SD) of the triplicates