Fig. 2

Production of 2,3-BD and lactate by Crabtree positive and negative S. cerevisiae. a Schematic representation of 2,3-BD and lactate biosynthetic pathways in S. cerevisiae. Both lactate dehydrogenase (LlLDH) from Lactococcus lactis and cytoplasm-relocated acetolactate synthase (cytoILV2) are shown in red, and yeast native steps, including spontaneous reaction and NAD-dependent (R,R)-butanediol dehydrogenase (BDH1) are shown in orange. b Titer of 2,3-BD produced by Crabtree positive 11C-BD and Crabtree negative 28-BD; c specific titer of 2,3-BD in Crabtree positive 11C-BD and Crabtree negative 28-BD; d titer of lactate produced by Crabtree positive 11C-LA and Crabtree negative 28-LA; e specific titer of lactate in Crabtree positive 11C-LA and Crabtree negative 28-LA. **p value < 0.005, *p value < 0.05