Fig. 4

Production of farnesene and lycopene by Crabtree positive and negative S. cerevisiae. a Schematic representation of farenese and lycopene biosynthetic pathways in S. cerevisiae. Yeast native genes including phosphomevalonate kinase (ERG8), acetyl-CoA C-acetyltransferase (ERG10), mevalonate kinase (ERG12), 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase (ERG13), farnesyl pyrophosphate synthetase (ERG20), HMG-CoA reductase (HMG1, HMG2), isopentenyl diphosphate: dimethylallyl diphosphate isomerase (IDI1), mevalonate pyrophosphate decarboxylase (MVD1), and geranylgeranyl diphosphate synthase (BTS1) are shown in orange. Farnesene synthase (MdFS) from Malus domestica, geranylgeranyl pyrophosphate synthase (PaCrtE) from Pantoea ananatis, phytoene synthase (PaCrtB) from Pantoea ananatis, and phytoene desaturase (PaCrtI) from Pantoea ananatis are shown in red. b Titer of farnesene produced by Crabtree positive 11C-Far and 28-Far. c Titer of lycopene produced by Crabtree positive 11C-Lyc and Crabtree negative 28-Lyc. *p value < 0.05