Fig. 1From: Simultaneous expression of an endogenous spermidine synthase and a butanol dehydrogenase from Thermoanaerobacter pseudethanolicus in Clostridium thermocellum results in increased resistance to acetic acid and furans, increased ethanol production and an increase in thermotoleranceEffects of BdhA expression on cell growth and fermentation products of C. thermocellum strains in defined medium without fermentation inhibitors (A and B) and containing 5 (C and D), 10 (E and F), or 15 (G and H) mM acetic acid. Strains were grown in defined medium with 5 g/L cellobiose containing 10 µg/mL thiamphenicol. A, C, E, and G Cell growth of the JWCT14 strain containing bdhA and pJGW37 plasmid compared to the control strain. B, D, F, and H Cellobiose consumed and fermentation products of JWCT13 and JWCT16 strains. JWCT13, parental strain containing pJGW37; JWCT14, BdhA expression strain containing pJGW37. Results are the mean of duplicate experiments and error bars indicate standard deviationBack to article page