Fig. 4From: Simultaneous expression of an endogenous spermidine synthase and a butanol dehydrogenase from Thermoanaerobacter pseudethanolicus in Clostridium thermocellum results in increased resistance to acetic acid and furans, increased ethanol production and an increase in thermotoleranceEffects of SpeE expression with the BdhA expression on growth of C. thermocellum at 65 °C. A and B Cell growth (A) and cellobiose consumed and fermentation products (B) of JWCT13 with and without 1 mM spermidine, JWCT14, JWCT15, and JWCT16 strains in defined medium with 5 g/L cellobiose containing 10 µg/mL thiamphenicol. JWCT13, parental strain; JWCT14, BdhA expressing strain; JWCT15, SpeE expressing strain; JWCT16, BdhA and SpeE expressing strain. Results are the mean of duplicate experiments and error bars indicate s.dBack to article page