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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Simultaneous expression of an endogenous spermidine synthase and a butanol dehydrogenase from Thermoanaerobacter pseudethanolicus in Clostridium thermocellum results in increased resistance to acetic acid and furans, increased ethanol production and an increase in thermotolerance

Fig. 4

Effects of SpeE expression with the BdhA expression on growth of C. thermocellum at 65 °C. A and B Cell growth (A) and cellobiose consumed and fermentation products (B) of JWCT13 with and without 1 mM spermidine, JWCT14, JWCT15, and JWCT16 strains in defined medium with 5 g/L cellobiose containing 10 µg/mL thiamphenicol. JWCT13, parental strain; JWCT14, BdhA expressing strain; JWCT15, SpeE expressing strain; JWCT16, BdhA and SpeE expressing strain. Results are the mean of duplicate experiments and error bars indicate s.d

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