Table 1 Strains and plasmids used in this study

Strains

 LL1005

C. thermocellum DSM 1313 pyrF (ura⁻/5-FOA^R)

[21]

 JWCT02

LL1005 containing pDCW89 (ura⁺/5-FOA^S)

[21]

 JWCT06

LL1005 containing pSKW01 (ura⁺/5-FOA^S)

[10]

 JWCT08-1

LL1005::P_enolase—bdhA containing pJGW37 (ura⁺/5-FOA^S)

[10]

 JWCT13

LL1005 containing pJGW37 (ura⁻/5-FOA^R/Tm^R)

[11]

 JWCT14

JWCT08-1 containing pJGW37 (ura⁺/5-FOA^S/Tm^R)

This study

 JWCT15

LL1005 containing pSKW59 (ura⁻/5-FOA^R /Tm^R)

[11]

 JWCT16

JWCT08-1 containing pSKW59 (ura⁺/5-FOA^S/Tm^R)

This study

Plasmids

 pDCW89

E. coli/C. thermocellum shuttle vector P_Cbes2105 -pyrF

[21, 22]

 pJGW37

E. coli/C. thermocellum shuttle vector P_Cbes2105 -cat

[21]

 pSKW01

Expression vector P_Cbes2105—pyrF and P_S-layer -bdhA

[10]

 pSKW59

Expression vector P_Cbes2105—cat and P_S-layer -speE

[11]