Fig. 1

Bioreactor cultivation of Rhodotorula toruloides CBS14 using as carbon source: a crude glycerol (CG), or b mixture of CG and hemicellulose hydrolysate (CGHH). The reconstruction of the cultivation curves was performed using results from [13]. Independent cultures, different from those whose values are the basis of the shown growth curves, were performed for RNA-isolation. At each sampling, concentrations of biomass, lipids and substrate were determined, to ensure the cells were in the same growth phase as indicated in the figure. Glycerol concentrations are given on the primary y axis. The secondary y axis indicates the concentrations of xylose, glucose and acetic acid in the hemicellulose hydrolysate. Vertical dashed lines represent sampling points for RNA extraction