Fig. 5
From: Respiration-based investigation of adsorbent-bioprocess compatibility
Cultivation of U. cynodontis NBRC9727 Δfuz7r Δcyp3r PetefmttA Pria1ria1 treated with different adsorbents according to Fig. 2 protocol step Ba, for investigation of nutrient adsorption. Depicted are A the oxygen transfer rate (OTR) and B the final itaconic acid concentration and optical density at 600 nm for one set of adsorbents suitable for itaconic acid adsorption. Detailed information about the applied adsorbents is shown in Table 1. Cultivations were performed in a RAMOS device at 30 °C, 350 rpm, VL = 10 mL in 250 mL RAMOS shake flasks at a shaking diameter of 50 mm, initial pH value 6, 25 g/L glucose in Verduyn medium. For clarity, only every tenth measuring point is marked as a symbol. Adsorbents, 10 mgadsorbent/mL, were added. B Bars for itaconic acid concentration on the left and for optical density on the right. For all curves, single measurements are shown