Fig. 3

Hydrolysis of cellulosic and hemicellulose substrates by TwCel5_CAT and TwCel5_CBM as a function of time. Reactions were performed in a 50 mM sodium phosphate buffer (pH 7.5) containing 0.5 M NaCl and 50 nM enzyme incubated at 30 ºC for 1 h in triplicates. Reducing sugar equivalents were quantified using glucose as a standard. The results correspond to mean and standard deviations of triplicates