Fig. 4

Analysis of hydrolytic products generated by TwCel5_CAT on different polysaccharides. A HPAEC-PAD chromatogram showing the soluble products generated upon 0.5% (w/v) PASC incubation of 1 µM enzyme in 50 mM sodium phosphate buffer (pH 7.5) at 30 °C for 24 h. B MALDI-TOF MS analysis of products generated from β-glucan (upper) and konjac glucomannan (lower) 50 nM enzyme incubation in 50 mM sodium phosphate, pH 7.5 and 0.5 M NaCl for 60 min at 30 °C. The lower panel shows the Na⁺ and K⁺ adducts of oligosaccharide, and ‘DP’ stands for degree of polymerization or ‘Ac’ for acetylation. None of the labelled peaks were observed in the negative control (i.e., a reaction without enzyme). Note the m/z difference of 162 observed between the peaks suggest a difference of a hexose residue. The products were identified based on cello-oligo standards, as shown